4069

3847

كارشناسي ارشد

بيماري شناسي گياهي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1386

يازده، 85، [II] ص.: مصور، جدول، نمودار

ص. ع. به فارسي و انگليسي

امير مساح، علي آهون منش

05/05/87

مسعود بهار، پرويز احسان زاده

1396/03/08

كتابنامه

كشاورزي

مهندسي كشاورزي

ID3847

به فارسي و انگليسي: قابل رؤيت در نسخه ديجيتال

AbstractMembers of the genus Polerovirus Luteoviridae that cause yellowing in sugar beetconsist of three species Beet mild yellowing virus BMYV Beet western yellows virus BWYV and Beet chlorosis virus BChV For detection and determination of certainmolecular properties of poleroviruses infecting sugar beet during 1385 1386 571 leafsamples of sugar beet with yellowing and thickening symptoms in older leaves werecollected from sugar beet fields at Isfahan and Charmahal o bakhtiary provinces Samples were tested using a polyclonal BWYV antibody in DAS ELISA technique None of samples in Chahar mahal o bakhtiary province were infected to polerovirusesof infecting sugar beet Of the 290 Isfahan samples tested 5 samples recognizedinfected to polerovirus by DAS ELISA RT PCR was conducted on the ELISA positivesamples using specific primers of beet poleroviruses The primer used to amplify ORF3 Coat protein gen of beet poleroviruses was CP and CP Three sets of primers wereused to amplify ORF0 of beet poleroviruses consisting of MpxBM and MpxBM MpxBW and MpxBW and MpxBC and MpxBC for specific detection of BMYV BWYV and BChV respectively Expected sizes of 563 bp and 441 bp were amplifiedusing CP and CP and MpxBM and MpxBM primers respectively These specificamplifications represent presence of BMYV in infected sugar beet samples The PCRproducts of an isolate named BMYV Lav were cloned and sequenced Multiplealignment of deduced amino acid sequence of ORF0 gene of BMYV Lav and those ofother poleroviruses infecting beet and rape revealed the highest similarity with BMYV 1 isolate 98 whereas analysis of amino acid CP gene showed the highest similaritywith a BWYV isolate 97 9 with Brassica yellow virus GB In phylogenetic analysisbased on amino acid sequence of ORF0 gene a gene at 5 end of genome BMYV Lavwas placed in BMYV isolates group whereas alignment of Coat protein CP gene agene at 3 end of genome of BMYV Lav showed that BMYV Lav isolate was closer toBWYV isolates than BMYV isolates These results suggested taking place ofrecombination in BMYV Lav

امير مساح، علي آهون منش

مسعود بهار، پرويز احسان زاده