تعيين نسبت ژنوم والدين وحشي و اهلي در هيبريدهاي گلرنگ با استفاده از نشانگر ملكولي ISSR

چكيده انگليسي :

Determination of Wild Genome Proportion in Wide Crosses of Safflower Using ISSR Markers Mohammad Taghi Rabbani mtrabbani@gmail com 1 3 2009 Department of Agricultural Biotechnology College of Agriculture Isfahan University of Technology Isfahan 84156 83111 Iran Degree MSc Language Farsi A Mirlohi Prof mirlohi@cc iut ac ir G Saeidi Prof gsaeidi@cc iut ac ir Abstract Safflower Carthamus tinctorius L 2n 2x 24 is one of the world s oldest oilseed crop with numerous past and present uses Similar to the main crops producibility limitations could be overcome by broadening the genetic base of breeding materials through interspecific hybridization Natural and artificial hybridization between safflower and several wild relatives have been reported The wild species of C oxyacantha Bieb syn C oxyacanthus 2n 2x 24 can be simply exploited to broaden the genetic base of safflower through interspecific hybridization This species has comparable including agronomic traits oil content and fatty acid composition and even resistance to safflower fly to cultivated safflower however there is lack of information on utilization of this species to enrich safflower gene pool Intersimple sequence repeats ISSR is a PCR based marker in which 16 25 bp long microsatellites prime in a single primer PCR reaction to amplify the inter microsatellite regions This technique has been extensively employed to estimate inter and intraspecific genetic variation in a wide range of crops and recently among cultivated safflower and its wild relatives In this research ISSR polymorphism was employed to study crossabilty of cultivated safflower C tinctorius L and wild relative C oxyacantha Bieb 2 determine the cultivated and wild parentals genome contribution into F2 progenies and 3 asses the efficiency of transferring parental specific markers in three crosses between two cultivated genotypes C4110 and C111 and one accession of wild species ISF2 using 25 ISSR primers Two spineless white seeded and red flowered genotype of cultivated safflower C4110 and C111 and a spiny yellow flowered and brown seeded accession of C oxyacantha ISF2 were used as parents in a paired reciprocal crosses established in 2007 F1 hybrids were detected on the basis of morphological characters and ISSR fingerprinting Three F2 populations with totally 140 progenies were obtained after intercrossing ten F1 hybrids A set of 25 ISSR primers capable of generating polymorphic and repeatable bands were used to amplify inter SSR sequences Two of used primers were anchored at 5 end while other 22 primers were anchored at 3 end To anchor to the 3 or 5 borders 1 to 3 selective nucleotides differing from the repeat motif were used Clearly visible polymorphic amplified DNA fragments resulted from ISSR analysis between parents and F2 individuals were scored Segregation analysis was checked against the expected 1 1 theoretical Mendelian ratio F2 2 population using a x test 1 df The average polymorphism for GA CT CA and GT microsatellites motif were 38 4 34 4 39 3 and 47 7 respectively In population resulting from cross between C4110 ISF2 of 193 parental specific markers maximum 138 and minimum 78 with an average of 122 3 parental specific bands were inherited from parents into progenies In populations resulting from ISF2 C4110 and C111 ISF2 of 192 specific parental markers maximum minimum and average of 162 101 and 137 3 and 152 109 and 133 bands were inherited respectively Results of this study showed that not all the parental markers have been transmitted to F2 progenies In C4110 ISF2 the efficiency of parental marker s inheritability for cultivated and wild parents was assessed 70 8 and 67 8 respectively In ISF2 C4110 the corresponding values were 73 and 73 3 respectively And in C111 ISF2 the efficiency of parental markers inheritability was 76 6 and 67 6 respectively Chi squar analysis showed a segregation distortion in cross C4110 ISF2 from 1 1 expected ratio at 0 05 error level And no skewed segregation wa

رباني، محمدتقي

تعيين نسبت ژنوم والدين وحشي و اهلي در هيبريدهاي گلرنگ با استفاده از نشانگر ملكولي ISSR

گلرنگ زراعي , گلرنگ وحشي , تلاقي بين گونه اي