4787

4506

كارشناسي ارشد

شيمي تجزيه

اصفهان: دانشگاه صنعتي اصفهان، دانشكده شيمي

1388

ده،79،[II]ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

محمد تقي جعفري، بهزاد رضايي

3/9/88

حسن قاضي عسگر، تقي خياميان

شيمي

ID4506

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Ion Mobility Spectrometry as a Detector for Molecular Imprinted Polymer Separation MIP IMS and Metronidazole Determination in the Pharmaceutical and Human Serum Bahareh Zaker Bahareh Zaker@gmail com Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc language farsiM T Jafari jafari@cc iut ac ir B Rezaei rezaei@cc iut ac ir Abstract In this work application of ion mobility spectrometry IMS as the detection technique for separationmethod based on molecular imprinted polymer MIP was investigated and evaluated for the first time Based on theobtained results MIP IMS system can be used for separation and detection of metronidazole drug in pharmaceuticaland human serum samples Ion mobility spectrometry IMS has many desirable characteristics to be integrated withMIP separations This technique is simple sensitive and portable which was developed for detection andcharacterization of chemical compounds The capability of IMS lets us identify sample molecules with the samemasses in addition to identical functional groups such as isomeric compounds Molecularly imprinted polymers MIPs are used as antibody like materials for high selectivity and sensitivity chemical inertness long termstability availability in large quantities and insolubility in water and most organic solvents The MIPs areextensively crosslinked polymers containing specific recognition sites with a predetermined selectivity for analytesof interest The molecular imprinting technique is a powerful method for preparing artificial recognition sites withpredetermined selectivity for a wide range of target molecules The procedure for synthesizing an MIP is based onthe polymerization of a functional monomer and a cross linking agent in the presence of a template When theimprinted molecule is removed the imprinted polymer with a high affinity for the template molecule is obtained This affinity is due to the shape and the arrangement of the functional groups of the monomer units The method isexhaustively validated in terms of sensitivity selectivity recovery reproducibility and column capacity The lineardynamic range of 0 05 70 00 mg L was obtained for the determination of metronidazole with IMS The recovery ofanalyzed drug was calculated to be above 89 and the relative standard deviation RSD was lower than 6 for allexperiments Various real samples were analyzed with the coupled techniques and the results obtained revealed theefficient clean up of the samples using MIP separation before the analysis by IMS as a detection technique Theresults obtained in this research showed that IMS is extremely compatible with these separations and the MIP IMSsystem has great capability for providing highly selective analysis of trace chemical and biochemical compounds Consequently based on this work results manual introduction of samples to the IMS is useful for showing potentialof this detection technique however an on line feeding of the MIP SPE elute into the IMS detector is necessary This work is being expanded to the analysis of various compounds such as drugs and pesticides in complexbiological and environmental extracts Keywords Molecular imprinted polymer Ion mobility spectrometry Metronidazole

محمد تقي جعفري، بهزاد رضايي

حسن قاضي عسگر، تقي خياميان