5249

4919

كارشناسي ارشد

تكثير و پرورش آبزيان

اصفهان: دانشگاه صنعتي اصفهان، دانشكده منابع طبيعي

1388

شانزده،88ص.:مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

سالاردرافشان،محمدپوركاظمي

يزدان كيواني،فريدون چكمه دوز قاسمي

18/3/89

عيسي ابراهيمي،منصور ملكيان

مهندسي منابع طبيعي

ID4919

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

107 Comparison of genetic variation of wild common carp Cyprinus carpio in the Anzali wetland using PCR RFLP Fariba Falahbagheri Fallah fariba@gmail com 2010 03 09 Department of Natural Resources Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Dr Salar Dorafshan Sdorafshan@cc iut ac ir Dr Mohammad Pourkazemi PKazemi m@yahoo com Abstract The Caspian Sea and its basin is one of the natural habitats of wild common carp Cyprinus carpio Considering the importance of the genetic variation and population structure studies for management and conservation of species the genetic diversity of wild common carp in the Anzali wetland was investigated using PCR RFLP technique In this study 200 sexually mature individuals of wild common carp were collected from five stations including Siah Keshim protected area Selke wild refuge Sorkhankol wild refuge Abkenar region and Anzali wetland estuary 40 individuals from each station DNA was extracted using ammonium acetate method from fin clips PCR amplification of the mtDNA D loop region was performed using a pair of primer and produced a fragment with 420bp length According to identifying this segment two specimens from Abkenar and Sea sequenced and submited in Gene Bank by accession number GU225730 and GU320667 respectively PCR product was digested using forty Endonuclease enzymes Enzyme digested pattern were observed using 6 polyacrilamid gel and silver staining Four enzymes TasI SmaI SspI ApoI showed polymorphism The results showed seven composite haplotypes among five stations Haplotypes AABA and ABAB were recognized specifically in Abkenar station and haplotypes BAAA and BAAB in Siah keshim and haplotypes BBBA and AAAB only observed in Sorkhankol station Genetic distances between haplotypes ranged from 0 01334 to 0 35000 Average haplotype and nucleotide diversity were 0 1321 and 0 014196 respectively Nucleotide divergence among population was measured as 0 0216 statistical Data analysis was performed using A2 test with 1000 interactions for detecting geographic heterogeneity This analysis showed significant differences between different haplotypes among populations except between Abkenar and Siah keshim stations pB0 5 Also the A2 value for Selke and the Caspian Sea estuary because of their similar haplotypes was zero This result showed that there is no difference between Selke and the Anzali wetland estuary stations It can be concluded that the PCR RFLP method in mtDNA D loop region is a suitable marker for population studies of the wild common carp in Anzali wetland and Caspian Sea estuary and this species in the region consists of at least three populations Abkenar Siahkeshim Sorkhankol and Selke Sea Key Word Wild common carp Cyprinus carpio PCR RFLP D loop Anzali wetland

سالاردرافشان،محمدپوركاظمي

يزدان كيواني،فريدون چكمه دوز قاسمي

عيسي ابراهيمي،منصور ملكيان