5274

4944

كارشناسي ارشد

بيوتكنولوژي كشاورزي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1388

يازده،72ص.:مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

بدرالدين ابراهيم سيدطباطبائي، رضا زارعي

احمد جعفري

22/3/89

سيروس قبادي،مجيد طالبي

مهندسي كشاورزي

ID4944

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Detection of chloroplast photosynthetic genes in punica granatum Tahereh Rezazadeh t rezazadeh@ag iut ac ir December 29 2009 Department of Agriculture Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi badraldin ebrahim sayed tabatabaei reza zareie sayedt@cc iut ac ir rzareie@cc iut ac ir Abstract Pomegranate is an economically important genus that includes valuable biochemical compounds where synthetized in chloroplast organel Therefore understanding the characterestics of plastid genes by molecular sequence analysis authorized us to elucidate mechanism of their production The chloroplast genome is 160 129 base pair in length and includes a pair of inverted repeats IR of 26 996 base pair separated by small and large single copy SSC LSC regions of 18 393 base pair and 87 744 base pair resepectively The psbl and psbf contribute in photosynthesis especially for functional process of photosystem These genes were aligned with their counterparts in 23 different plants which were exist NCBI gene bank According to this comparison PCR primers were selected using the primer selection computer program oligo 5 Finally the genes were amplified with selective primer pairs and were sequenced On the basis of the results the aligned part of psbl and psbf genes had a length of 117 and 120 bases respectively which encode two significant proteins comprising 38 and 39 amino acid residues Psbl and psbf genes had a ACG and ATG as a first codon respectively The alignment of the psbl and psbf nucleotide sequences from 26 plants shows strong conservation According to the results and analyzing the sequencing patterns of 3 groups of pomogarante genus which encompasses wild ornamental and domesticated ones with MEGA 4 software the relationship among 26 genotypes represented by the Minimum Evolution dendrogram In the phylogenetic tree wild ornamental and domesticated genotypes of pomegranate genus completely separated and classified into three distinct groups Moreover domesticated genotypes were grouped closer to oenothera pariviflora strengthening the presumption that from the close relationship of these genotypes resulted from locating them

بدرالدين ابراهيم سيدطباطبائي، رضا زارعي

احمد جعفري

سيروس قبادي،مجيد طالبي