5291

4961

كارشناسي ارشد

بيماري شناسي گياهي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1388

يازده،131ص.:مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

بهرام شريف نبي

ناصر صفايي، ابوالقاسم اسماعيلي

29/3/89

امير مساح،مجيد طالبي

1396/10/03

كتابنامه

مهندسي كشاورزي

ID4961

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Detection of Genes Involved in Destruxin B Production and the Role of Genes in Alternaria brassicae Pathogenicity in Rapeseed Masumeh Mostafa Mostafai2006@yahoo com Date of submission March 23 2010 Department of Plant Protection Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi B Sharifnabi Ph D Assoc Professor Supervisor Sharifna@cc iut ac ir Abstract Alternaria leaf spot is one the most important fungal disease of Crusiferous palnts that affect the quality and quantity of these plants every year This disease is caused by three seedborne pathogenic fungi named A brassicae A brassicicola and A raphani in most area of the world Infection caused by Alternaria spp is an important in the decrease of the seed germination A brassicae is known as disease agent of canola leaf spot in Canada America India and most Euroupian countries and in Iran this species was isolated from canola fields of Golestan Khozestan and western Azarbayejan A brassicae produce some phytotoxins in infected tissue and followed by the colonization of infected tissues A brassicae produce some phytotoxins in host tissue Destruxin B is the major phytotoxin produced by this fungus in vitro and in plant Synthesis and secreting of this toxin is related to NRPS ABC transporter gene cluster in the genom of thes fungus Mor gene analysis of this cluster my show the role of NRPS ABC transporter gene cluster and phytotoxins synthesis by them in pathogenicity ecology and physiology Therfore this research focused on the study of disease mechanism in six isolates of A brassicae by molecular chemical and biological methods to increase our knowledge of disease mechanism In this research from 65 isolates of Alternaria spp isolated from leaf pod and stem of canola was obtained four species A alternate A arborescense A infectoria and A raphani six isolates of A brassicae was taken from Tarbiat Modarres of Tehran A brassicae isolates confirmed by the use of specific primers For detecton of present or absence of AbrePsy1 and AbreAtr1 genes in the studied isolates PCR assay was done The amount of transcription in the mentioned genes correspondenced to pathogenicity NRPS ABC transporter gene was studied by Real time PCR method In determining the ability of toxin production produced toxin was extracted and relatively purified The role of phytotoxins produced by each isolate was investigated on canola plants in vitro On the other hand severity of pathogenicity of each isolate was measured in the greenhouse For assessment of AbrePsy1 and AbreAtr 1 expression and the confirmation of the their role in pathogenecity of A brassicae the correlation between the amount of transcription the amount of phytotoxicity and the amountof pathogenicity in the isolates were also studied The results showed that the capacity of the phytotoxin production and pathogenicity in A brassicae is more related to the amount of transcription of AbrePsy1 gene in this funus as the role of this gene was not studied previously and the role of NRPS was shown in pathogenicity On the basis the results it was revealed that amount of AbreAtr1 expression can indirectly effect the expression of AbrePsy1 genes that in the following the production of phytotoxin and amount of pathogenicity is indirectly effected of the action ABC transporter Keywords canola A brassicae Phytotoxin NRPS ABC transporter PDF created with pdfFactory Pro trial version www pdffactory com

بهرام شريف نبي

ناصر صفايي، ابوالقاسم اسماعيلي

امير مساح،مجيد طالبي