پديد آورنده :
يحيائي، محمد
عنوان :
بررسي ميزان بيان ژن TNFR_1 و ارتباط آن با نرخ تخمك ريزي در 4 نژاد گوسفند با عملكرد توليد مثلي متفاوت
مقطع تحصيلي :
كارشناسي ارشد
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
يازده،82ص.:مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
حميدرضا رحماني،محمد خوروش
استاد مشاور :
محسن فروزان فر، مهدي محمد عليپور
توصيفگر ها :
سلول هاي گرانولوزا , فوليكول آنتروم دار , آترزيا
تاريخ نمايه سازي :
7/4/89
استاد داور :
سعيد انصاري،مهدي رحيم ملك
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Study of TNFR Gene Expression and its Relation with Ovulation Rate in 4 Genotypes of Sheep with Different Reproductive Performances Mohammad Yahyaei M yahyaei2008@gmail com March 16 2010 Department of Animal Science Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi H R Rahmani Supervisor hrahmani@cc iut ac ir Abstract The aim of the present study was to examine the influence of TNFR mRNA expression on death of granulose cells of antrum follicle and its relation with ovulation rate in sheep One breed with relatively high ovulation rate Shall and another with relatively low ovulation rate Lori Bakhtiari were compared regarding Their granulose cells expression of TNFR Ewes fed similar and not using of any method to enhance ovulation rate Estrus was synchronized in all animals with the CIDER 12 day The granulose cells were collected from antrum follicle 48 hourse after CIDER withdrawal Total RNA was extracted from cells using an RNeasy mini kit Qiagen and then reverse transcribed to synthesize cDNA The expression of TNFR in both breeds was analyzed by semiquantitative RT PCR TNFR was not expressed in granulose cells of antrum follicle in sheep The current study demonstrates that TNF act as a survival factor or death factor in a follicular stage dependent manner antrum follicle atresia is one of the important mechanisms for ovulation rate in female mammals and TNFR plays an important role in the control of follicular atresia also ovulation rate in the Shall breed is not controled by major genes reported booroola Hana Inverdale Cambridge our results indicate that TNFR is not the major factor responsible for shall and lori Bakhtiari differences in ovulation rate Key word granulose cell breed antral follicle ovulation rate atresia
استاد راهنما :
حميدرضا رحماني،محمد خوروش
استاد مشاور :
محسن فروزان فر، مهدي محمد عليپور
استاد داور :
سعيد انصاري،مهدي رحيم ملك