پديد آورنده :
خورشيديان، مجيد
عنوان :
بهينه سازي شرايط استخراج و بازيابي آنزيم گلوكز آميلاز با استفاده از نانو قطرات مايسل معكوس
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
مهندسي شيمي
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده مهندسي شيمي
صفحه شمار :
چهارده،98ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
حميد زيلويي، مرتضي خان احمدي
استاد مشاور :
كيخسرو كريمي
توصيفگر ها :
سي تب , خالص سازي پروتئين
تاريخ نمايه سازي :
13/7/89
استاد داور :
محمدرضا احساني، محمد فضيلتي
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Optimized Forward and Backward Extraction of Glucoamylase Using Nanoparticulate Reverse Micelles Majid Khorshidian m khorshidian@ce iut ac ir Date of Submission 2010 04 24 Department of Chemical Engineering Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisors H Zilouei hzilouei@cc iut ac ir M Khanahmadi khanahmadi@yahoo comAbstractGlucoamylase GA enzyme has been got wide application in the food and fermentation industries It has been gotten second position in world s distribution and sales of industrial enzymes In thisstudy extracellular enzyme GA from Aspergillus niger produced using solid state fermentation was separated by nanoparticulate reverse micelles of CTAB cationic surfactant A reverse micelleextraction cycle is basically composed of two processes forward and backward extraction In theforward extraction step enzymes are transferred from the initial aqueous phase to the reversemicelles and in back extraction step enzyme are transferred from the reverse micelles back to afresh aqueous strip solution The effects of surfactant concentration pH NaCl concentration cosolvent concentration phase volume ratio and surfactant charged kind on the both steps ofextraction efficiency were tested Following single factor experiment the optimum extraction wasachieved by response surface methodology RSM using central composite rotatable design CCRD The experimental results concluded the highest forward extraction efficiency of GAactivity at pH 8 NaCl concentration 50 mM CTAB concentration 100 mM and cosolventconcentration 10 Moreover for the maximum back extraction recovery of the enzyme pH andNaCl concentration were obtained to be 4 2 and 417 mM respectively Finally by the optimizationof phase volume ratio for both steps purification factor and efficiency of enzyme recoveryincreased from 2 15 and 73 to 2 7 and 76 respectively This study demonstrated that liquid liquid extraction by CTAB reversed micelles is an efficient process to separate and purify the GAfrom fermentation broth Keywords Nanoparticulate reverse micelles CTAB glucoamylase protein purification
استاد راهنما :
حميد زيلويي، مرتضي خان احمدي
استاد مشاور :
كيخسرو كريمي
استاد داور :
محمدرضا احساني، محمد فضيلتي
