پديد آورنده :
خليلي، بنفشه
عنوان :
مطالعه قارچ هاي سلولايتيك، الگوي ايزو آنزيمي و سنجش ثابت هاي سينتيكي و ترموديناميكي آنزيم سلولاز در خاك هاي متاثر از تغيير كاربري
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
سيزده،107ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
فرشيد نوربخش، نفيسه نيلي
استاد مشاور :
بهرام شريف نبي، حسين خادمي
توصيفگر ها :
قارچ , كربن , جنگل، مرتع و بيابان
تاريخ نمايه سازي :
27/9/89
استاد داور :
آقا فخر مير لوحي، اميرلكزيان، كاظم خاوازي
كد ايرانداك :
ID339 دكتري
چكيده فارسي :
به فارسي و انگليسي: قابل رويت درنسخه ديجيتالي
چكيده انگليسي :
Cellulytic Fungi Diversity Isoenzyme Pattern and Kinetic and Thermodynamic Constants of Soil Cellulase as Influenced by Land Use Change Banafshe Khalili E mail address b khalili@ag iut ac ir October 27 2010 Department of Soil Science Isfahan University of technology Isfahan 84156 83111 Iran Supervisor Farshid Nourbakhsh E mail address farshid@cc iut ac ir Supervisor Nafise Nili E mail address nilin@cc iut ac ir Advisor Bahram Sharifnabi Advisor Hossein Khademi Department Graduate Program Coordinator Farshid Nourbakhsh Department of Soil Science College of Agriculture Isfahan University of Technology Isfahan Iran Department of Biotechnology College of Agriculture Isfahan University of Technology Isfahan Iran Abstract This study was conducted to determine how cultivation influences the activity and diversity as well as kinetic and thermodynamic parameters of soil cellulase in three ecosystems including forest rangeland and saline desert soils Land use change effects on the amount and diversity of soil proteins were also studied Soil samples were collected in October 2009 from a native forest a native rangeland and a native saline desert as well as adjacent cultivated fields in central Iran Land use changes not only decreased the amount of soil proteins but also influenced the protein pattern Soil protein content decreased by 64 55 and 90 in the cultivated sites as compared with their corresponding native forest and native rangeland sites respectively Electrophoretic patterns of soil proteins from agricultural soils were clearly different from those of the native lands Cultivation of the native forest rangeland and saline desert soils resulted in 79 47 and 73 reductions in cellulase activity respectively The Michaelis constant Km increased but the maximum catalytic velocity Vmax decreased for soil cellulase as a result of cultivation in the study areas The thermodynamic parameters Ea and Q10 of soil cellulase were also affected by cultivation Higher Ea and Q10 values were obtained for the native soils but the lower values were observed in the cultivated soils The cellulase zymographic pattern revealed different cellulase isoenzymes in the soils The results confirm the hypothesis that differences in the kinetic and thermodynamic parameters of soil enzymes could be due to the presence of different isoenzymes Key WordsCellulase land use change protein diversity isoenzyme kinetic and thermodynamic parameters fungal diversity
استاد راهنما :
فرشيد نوربخش، نفيسه نيلي
استاد مشاور :
بهرام شريف نبي، حسين خادمي
استاد داور :
آقا فخر مير لوحي، اميرلكزيان، كاظم خاوازي
