بررسي بيان ژن CGRP و عملكرد كبد گاوهاي شيري تغذيه شده با مكمل چربي طي دوره انتقال

چكيده انگليسي :

Assessing of CGRP Gene Expression and liverfunction of Dairy Cattle Fed with Fat Supplement in Transition Period Hamideh Keshavarzi h keshavarz 313@Yahoo com Date of Submission 2 Novamber 2010 Department of Animal Science Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisor M A Edriss edriss@cc iut ac ir Abstract In the higher standards of milk production the priority in modern breeding is keeping dairy cows in high lactation and healthy It is known that highly productive animals compared to those who produce less are in a more or less negative energy status The negative energy status mostly appears during three situations in late pregnancy in the first weeks of lactation and during disease Thus priority for higher milk production is prevention of metabolic disorders and other diseases According to animal nutrition experts to prevent a negative energy balance particularly during the transition period fat supplement can be used Fat supplements used in this study granulate fat soybean meal that was added to the concentrate part This study was performed at the Animal Husbandary Unit College of Agriculture Isfahan University of Technology located 40km western south of Isfahan Iran Twenty four two and multiparous Holstein cows with mean body weight 700 50 kg in first period were used in a randomized complete design experiment to determination effect of Supplemental fat on gene expression composition and milk yield and liver function in transition period Cows before calving were divided into two group and one group received control diet include 73 3 corn silage 7 2 alfalfa hay 2 wheat straw and 20 5 concentrate and another group fat supplement include 59 5 corn silage 8 4 alfalfa hay 8 6 wheat straw and 23 5 concentrate include 2 5 fat soybean meal In after parturition cows divided two group again and one group received control diet include 65 5 corn silage 5 5 alfalfa hay and 34 concentrate and another group fat supplement include 59 corn silage 7 5 alfalfa hay and 33 5 concentrate include 3 5 fat soybean meal as cows under experiment were included four groups 1 Group fed with control diet pre and postpartum 2 Group fed with control diet pre and fat diet postpartum 3 Group fed with fat diet pre and control diet postpartum 4 Group fed with fat diet pre and postpartum Milk production was recorded at each milking Samples were obtained the end of every week during the experimental period and analyzed for determination milk component Blood samples were obtained before morning feeding as weekly of 14d before the expected day of parturition until 28 d after parturition Data were analyzed using repeated measures of the mixed procedure of SAS In this study was assessed calcitonin gene related peptide CGRP gene expression and It was found that this gene in liver tissue of dairy cows up to 60 days of lactation the ability of expression but is probably based on the research findings in liver tissue bulls and cows dry period is capable of expression As variety of amino acids this gene had most homology in cattle with the sheep mice rats and dogs thus if needed can be determined as this factor in the blood bovine with human or rat kit Fat supplements alter serum biochemical parameters but these changes were in the range of physiological and can conclude that cows under experiment were of Clinicaly healthy These results showed that adding fat supplements especially in the period before parturition without a negative effect on hematologic parameters and reduce liver function can increase cattle performance Key Words Fat supplement CGRP Gene Dairy cattele Transition period Liver function

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بررسي بيان ژن CGRP و عملكرد كبد گاوهاي شيري تغذيه شده با مكمل چربي طي دوره انتقال

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