5761

5376

كارشناسي ارشد

علوم و صنايع غذايي

اصفهان:دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1389

چهارده،123ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

مهدي كديور، امير حسين گلي

محمد فضيلتي

28/1/90

جواد كرامت، محمدرضا سبز عليان

1395/08/23

مهندسي كشاورزي

ID5376

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Process Optimization Using Response Surface Methodology to Produce Diacylglycerol Oil From Safflower Oil by Enzymatic Glycerolysis Sayed Mohammad Sahafi Sm sahafi@yahoo com March 13 2011 Department of Food Science and Technology Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Mahdi Kadivar Kadivar@cc iut ac ir Sayed Amir Hossein Goli Amirgoli@cc iut ac ir Abstract Oils and fats have been important components of human diet from very ancient times They are considered as sources of energy essential fatty acids and fat soluble vitamins and have key role in metabolism Today it has been proved that some fats and oils have also functional properties in human body and positive impact on human health These compounds are known as functional nutritional medical or pharmaceutical lipids According to differences in stages of digestion absorption and metabolism of diacylglycerol oil DAG oil in body and its favorable effects in preventing many diseases it seems that this oil is a good option for production as functional oil Diacylglycerols DAGs are esters of glycerol with two fatty acids FFAs which have two structural isomers i e sn 1 2 2 3 DAG and sn 1 3 DAG By definition DAG oil is an oil containing at least 10 DAG whereas commercial one contains 80 DAG maximum 20 triacylglycerol TAG and 5 monoacylglycerol MAG In 1999 for the first time this oil was produced from soybean and canola oils in Japan Econa oil In 2000 and 2003 this oil was registered as a GRAS Generally Recognized As Safe compound by the FDA Food and Drug Administration in USA Also in both countries DAG oil referred as functional cooking oil DAG oil can be produced with various routes such as hydrolysis glycerolysis and esterification or combination of these methods Each of these production methods can be done with alkaline inorganic chemical or enzyme catalysts In this study DAG oil was produced from safflower oil by enzymatic glycerolysis in solvent free reaction system Lipozyme TL IM was used as biocatalyst which is an immobilized 1 3 position specific lipase from Thermomyces lanuginosa Different variables including temperature time mole ratio of glycerol to oil and enzyme percentage were considered in oil production Finally optimization of the reaction and determination of optimum levels for each variable was done using response surface methodology RSM The results showed that there was no significant difference between the amount of DAG oil predicted by the software and its actual content and the model could well be used for production of desired amount of DAG Optimal conditions were found to be 0 75 enzyme 5 3g glycerol temperature of 46 9 C and reaction time of 4 h Finally physicochemical properties of DAG oil and safflower oil raw material were compared and results suggested that despite of slight differences in some properties DAG oil could be used as a replacement for safflower oil and even other conventional oils in food industry Key words Diacylglycerol oil Enzymatic glycerolysis Safflower oil Response surface methodology

مهدي كديور، امير حسين گلي

محمد فضيلتي

جواد كرامت، محمدرضا سبز عليان