5878

5493

كارشناسي ارشد

بيوتكنولوژي كشاورزي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1389

سيزده،76ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

بدرالدين ابراهيم سيد طباطبائي، مجيد طالبي

14/2/90

مسعود بهار، احد يامچي

مهندسي كشاورزي

ID5493

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Study of Phylogenetic Relationships in Pistacia Genus Using Chloroplast DNA Maryam Zamani m zamaniforoshani@ag iut ac ir Date of submission February 27 2011 Department of Agricultural Biotechnology Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi 1 B E Seyed Tabatabaei Professor Supervisor Sayedt@cc iut ac ir 2 M Talebi Ph D Assist Professor Supervisor mtalebi@cc iut ac ir Abstract The genus Pistacia is a member of the Anacardiaceae family and consists of at least 11 species Pistacia species are dioecious and wind pollinated trees Among these species Pistacia vera Pistachio has edible nuts and economically important species The other species usually grow wild and are used as rootstock for P vera P vera is native to north Afghanistan northeast Iran and central Asian republics Pistachio is an important horticultural and economical plant whose cultivation and production has a long history in Iran and the country is well known internationally for this interprise Iran has been considered as one of the origins of this plant The systematic position and intrageneric relationships of Pistacia species are controversial therefore precise genetic relationships among cultivars and species using molecular DNA markers is necessary to make better breeding programs Chloroplast DNA cpDNA has been used extensively to analyse plant phylogenies at different taxonomic levels because of conservative size organization and sequence Thus in this study in order to investigate the evolutionary trend of the genus Pistacia by using the plastid trnC trnD and atpB rbcL In this study plant material from commercially grown Pistachio cultivars was collected from four different countries including Iran Syria Turkey and United States of America TrnC trnD region was amplified with three pairs of specific primers The PCR products ligated into T vector and then transferred into E coli MC1061 strain with three method Cloning kit Electroporation and Calcium Clorid Clonies containing our fragments were selected by Colony PCR and Digestion procedures After sequencing three microsatellites were recognized in trnC trnD region and primer design from flanking region of the reapeats could introduce chloroplast microsatellite in Pistacia species The combined plastid data sets were analysed using MEGA4 software with maximum composite likelihood method Based on the data sets derived from the two regions of trnC trnD and atpB rbcL wild genotypes were separated from P vera cultivated species including cultivated genotypes from different countries and clustered in one group Clustering of Sarakhs with cultivated genotypes might be an indication that Sarakhs has contributed to the development of cultivated varieties In addition close relationship between Iranian cultivars and foreign types strengthen the presumption that Iran is one of the two major

بدرالدين ابراهيم سيد طباطبائي، مجيد طالبي

مسعود بهار، احد يامچي