تهيه و ارزيابي نانوالياف مخلوط پلي كاپرولاكتون حاوي سيمواستاتين و نانو ذرات شيشه زيست فعال جهت رهايش كنترل شده دارو در بازسازي استخوان

چكيده انگليسي :

Production and Evaluation of Polycaprolactone Blend Nanofibers Containing Simvastatin and Bioactive Glass Nano Particles for Controlled Drug Release in Bone Regeneration Monireh Kouhi Monireh Kouhi@gmail com Date of Submission 2011 3 9 Department of Textile Engineering Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisor s Names and Email addresses Mohammad Morshed morshed@cc iut ac ir Jaleh Varshosaz varshosaz@pharm mui ac ir Abstract These days nanofibrous materials are gaining great applications in biomedical fields including drug release device and tissue regeneration matrices For hard tissue regeneration composite nanofibers are favored due to their mechanical and biological properties Incorporation of bioactive ceramics such as calcium phosphates and bioactive glasses in composite nanofiber has largely demonstrated better performance in cellular growth bone cell differentiation and mineral deposition Moreover use of biopolymer as a composite matrix allows easy fabrication and provides a level of mechanical flexibility Among the inorganic phases bioactive glasses are a fascinating choice due to their excellent bioactivity to form a bone mineral like hydroxyapatite phase on the material surface It was shown that incorporating drug into nanocomposite has improved the efficacy of implants Simvastatin is one of the members of the statin family of drugs that known for lowering serum cholesterol and it has been reported that simvastatin stimulate bone formation In this thesis poly caprolactone PCL poly ethylene glycol PEG polymer blend nanofibrous web containing Bioactive Glass nanoparticle BG and Simvastatin drug has been produced to make a drug release device PCL PEG nanofiber was successfully fabricated by electrospinning technique from polymer blend solution containing different amounts of BG 0 20 and different ratios of PEG in the mixture of chloroform methanol 75 25 v v as solvent Different percentage of Simvastatin was added just before the electrospinning 0 6 6 and 12 Morphological and mechanical characteristics of nanofibrous web were evaluated Result showed that viscosity of polymer solution increased by increasing the amount of BG which led to an increase in the average fiber diameter observed by SEM moreover viscosity was decreased with an increase in the ratio of PEG and consequency nanofibers with smaller and less uniform diameter were produced Result showed that the amount of drug has no significant effect on nanofiber diameter Tensile stress was dependent on the amount of BG By increasing the percentage of BG from 0 to 15 tensile stress was significantly increased however there is a decrease in the tensile stress by increasing the amount of BG up to 20 which is probably due to agglomeration of BG in polymeric nanofibers DSC analysis was utilized to investigate the effect of BG on the crystallinity and structural properties of nanofiber and also the probable chemical interaction of polymer and drug DSC result showed that by increasing the amount of BG crystallinity of polymeric nanofiber was increased these result also showed that the melting peak of the drug disappeared in the nanofiber containing drug that showed drug molecules are in the amorphous form in the nanofiber Result of degradation tests indicated that the presence of BG and the addition of PEG increased the degradation rate of nanofibrous web that was due to an increase in its hydrophilicity Drug release rate was studied in the phosphate buffer medium with pH 7 4 the amount of drug dissolved in this medium was determined by using the UV spectrophotometery Results showed that the drug release rate was affected by the amount of the BG also the ratio of PEG By increasing the amount of BG initial release rate was decreased but the amount of drug that was released in prolonged time was increased also by increasing the PEG ratio the release rate was increased that is because of increase in nanofiber biodegradab

كوهي، منيره

تهيه و ارزيابي نانوالياف مخلوط پلي كاپرولاكتون حاوي سيمواستاتين و نانو ذرات شيشه زيست فعال جهت رهايش كنترل شده دارو در بازسازي استخوان

نانو الياف كامپوزيتي , رهايش دارو