6056

5666

كارشناسي ارشد

بيماري شناسي گياهي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1389

پانزده،104ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

بهرام شريف نبي

سيما زنگنه

21/4/90

امير مساح، فرشيد نوربخش

مهندسي كشاورزي

ID5666

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Identification of Olive Endomycorrhizal Fungi VAM and Their Interactions with Verticillium Wilt in Two Major Olive Cultivars Mohamamd Imad Khreibeh imadkhrieba@yahoo com Department of Plant Protection Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi B Sharifnabi Assoc Professor Supervisor sharifna@cc iut ac ir Abstract Verticillium wilt disease of olive is one of the most important diseases on olive tree considered inthe cultivated areas and is found world wide Reviews related to the biological control of Verticillium wiltusing fungi as antagonists showed that only pathogen antagonists have been introduced in the laboratoryand greenhouse and field assays are limited to species of genera Gliocladium Talaromyces Trichoderma and Arbuscular mycorrhiza fungi AMF Inoculated olive seedlings with mycorrhizal fungus Glomusmosseae in soil contaminated with V dahliae reduced disease severity and increased the growth of oliveseedlings In this study four species of endomycorrhizae Paraglomus occultum Glomus etunicatum G fasciculatum and G clarum isolated from rhizosphere soil of olive orchards in Giluan Province Loshan Manjil and Rudbar on the developmental process of interaction between mycorrhizal fungi andVerticillium wilt of olive was evaluated In the first stage causal agent factor Verticillium wilt of olivetrees isfahan was isolated and purified Based on the morphological and molecular studies Verticilliumdahliae kleba was identified This is the first report of isolation of V dahliae of the olive in isfahanprovince In the second stage of separation and purification of mycorrhizal fungi using sieving of the soilsuspension in water was done and in the third stage of inoculation mycorrhizal fungi inoculated to plantsby Sorghum pot culture method In the fourth stage mixing of Verticillium with mycorrhizal fungiinoculum with soil was performed of olive seedlings transplanting was done The effect of mycorrhizalfungi on olive Verticillium wilt disease traits leaf number branch number plant height fresh weight anddry olive branches and seedlings in the statistical factorial design with four treatments in a greenhouse sterilized soil only control sterilized soil mycorrhiza mycorrhiza sterilized soil and Verticillium Verticillium sterilized soil on olive Roghani and Zard varieties were studied The results showed thatmycorrhizal fungi in the presence of Verticillium increased fresh weight and dry weight of olive branchesand seedlings It also increases the number of leaves and branches of olive seedlings compared to controltreatment and Verticillium treatment in the absence of mycorrhiza Statistical calculations and analysis ofvariance showed a significant differences between treatments observed 0 05 P and were grouped basedon LSD test was performed Mycorrhizal fungi alone and in the absence of Verticillium in soil revealed apositive effect on traits in comparison to control It seems that mycorrhizal fungi could be used as anappropriate solution to reduce the degree of olive Verticillium wilt disease Key words Olive Verticillium wilt Vesicular Arbuscular mycorrhiza VAM Greenhouse

بهرام شريف نبي

سيما زنگنه

امير مساح، فرشيد نوربخش