پديد آورنده :
پاپ زن، زهرا
عنوان :
جداسازي، همسانه سازي و بيان هترولوگ سه ژن كد كننده ي تيوردوكسين نوع ( Ostrxh23 OsTrxh20 OsTrxh1 ﴾از گياه برنج Oryza sativa
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
بيوتكنولوژي كشاورزي
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
شانزده،139ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
آذر شاه پيري
توصيفگر ها :
بيان ژن , كروماتوگرافي جذبي , فعاليت كينتيكي , درخت فيلوژنتيك
تاريخ نمايه سازي :
25/4/90
استاد داور :
آقا فخر مير لوحي، حسن محبت كار
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Isolation Gene Cloning and Heterologous Expression of Three Genes Encoding Thioredoxin Type h OsTrxh1 OsTrxh20 and OsTrxh23 from Rice Oryza Sativa Zahra Papzan z papzan65@gmail com z papzan@ag iut ac ir Date of Submission March 05 2011 Department of Agricultural Biotechnology Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiA Shahpiri Assist Prof Supervisor a shahpiri@cc iut ac irAbstract Thioredoxins are small ubiquitous proteins with a redox active disulfide bridge present in the characteristicactive site sequence WCG PPC These proteins can participate in thiol disulfide reactions via two cysteinesresidues of their active site motif They have a molecular mass of approximately 12 14 kDa and areuniversally distributed in all organisms from prokaryotes to higher eukaryotes Plants contain several formsof Trxs Thioredoxin f m x and y are found in the chloroplast Trxo is localized in mitochondria and Trxh istypically cytosolic Trxh have been found to have an important role in plants development In cytosol electrons flow from NADPH through Trxh by NADPH Thioredoxin Reductase NTR A total of 30 loci with54 gene models encoding putative Trx proteins were identified in the Rice genome The number of 9 genesare Trxh encoding genes Despite of widespread studies on NTR Trx system in plants the function of eachisoform had not been characterized yet In the present study the gene encoding OsTrxh23 was isolated fromshoots of Oryza sativa L2 seedlings 7 days after germination by Reverse Transcriptase PCR RT PCR method and then the RT PCR products was cloned in to pJET cloning vector In order to producerecombinant protein the gene was cloned in to pET 15b expression vector which contains T7 promoter and
استاد راهنما :
آذر شاه پيري
استاد داور :
آقا فخر مير لوحي، حسن محبت كار
