پديد آورنده :
صحيحي، مهدي
عنوان :
پايداري ترموديناميكي و خواص پيوندي بتالاكتوگلوبولين ﴿BLG﴾
گرايش تحصيلي :
شيمي فيزيك
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده شيمي
صفحه شمار :
هجده،163ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
يوسف غايب، عبدالخالق بردبار
استاد مشاور :
تقي خياميان
توصيفگر ها :
سوزفكتانت هاي يوني , غير طبيعي شدن , اتصال رتينول , اتصال مولكولي , شبيه سازي ديناميك مولكولي
تاريخ نمايه سازي :
26/1/91
استاد داور :
اميرعباس رفعتي، داود عاجلو، عزت كشاورزي
كد ايرانداك :
ID421 دكتري
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
172Thermodynamic Stability and Binding Properties of Lactoglobulin Mehdi Sahihi msahihi@ch iut ac ir September 28 2011 Department of Chemistry Isfahan University of Technology 84156 83111 Isfahan IranDegree Ph D Language FarsiSupervisors Dr Yousef Ghayeb E mail yghayeb@gmail comProf Abdol Khalegh Bordbar E mail bordbar@chem ui ac irAdvisor Prof Taghi Khayamian E mail taghi@cc iut ac irDepartment Graduate Program Coordinator Prof Bijan NajafiAbstractIn this work the stability parameters of bovine beta lactoglobulin variant A BLG A with regard to theirtransition curves induced by cetylpyridinium chloride CPC dodecyltrimethylammonium bromide C12TAB tetradecyltrimethylammonium bromide C14TAB hexadecyltrimethylammonium bromide C16TAB and sodium dodecylbenzenesulfonate SDBS as ionic surfactants were determined at 298 K Foreach transition curve the conventional method of analysis which assumes a linear concentration dependenceof the pre and post transition base lines gave the most realistic values for 8GD H2O Subsequently theretinol binding was investigated by BLG in the presence of various amounts of these surfactants as itsextrinsic functional binding fluorophore The comparison of the results allowed for determining the bindingof retinol by BLG in the presence of these ionic surfactants Also the interaction of resveratrol as a naturalpolyphenolic compound bisdemethoxycurcumin BDMC and diacetylbisdemethoxycurcumin DABC asthe bioactive constituents of turmeric with BLG was studied using molecular docking and moleculardynamics simulation methods Molecular docking studies revealed that the resveratrol BDMC and DABCwere bound to the surface of the protein by two four and five hydrogen bond interactions respectively Furthermore the results of molecular dynamics simulation represented that complexation of BDMC to BLGwill change the conformation of BLG Key Words Lactoglobulin Ionic Surfactants Denaturation Retinol Binding Property Molecular Docking MolecularDynamics Simulation
استاد راهنما :
يوسف غايب، عبدالخالق بردبار
استاد مشاور :
تقي خياميان
استاد داور :
اميرعباس رفعتي، داود عاجلو، عزت كشاورزي
