6838

6380

كارشناسي ارشد

علوم دامي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1390

سيزده،74ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

حميدرضا رحماني، محمد خوروش

محمد علي ادريس

18/4/91

غلامرضا قرباني، بدرالدين ابراهيم طباطبائي

مهندسي كشاورزي

ID6380

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Nano Polymer Coating Insulin IGF I and Their Absorbtion Evaluationin Duodenum of Sheep Shiva Oliya s oliya@ag iut ac ir December 2011 Department of Animal Science Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi H R Rahmani Prof Supervisor hrahmani@cc iut ac irAbstractIn the present study two experiments were carried out In the first experiment insulin coated using Nano polymer in order to calculate its duodenum absorption in sheeps Scine the parenteral administration is theonly route of insulin delivery alternative route of administration oral nasal rectal pulmonary andocular have been extensively investigated among them the oral route seems to be the most convenientand physiological because insulin undergoes a first hepatic glucose output However insulin is stronglydegraded by proteolytic enzyme in the gastrointestinal tract In addition this peptide is very poorlyabsorbed after oral administration In order to protect it from biodegradation and to improve its intestinalabsorption insulin has been associated to antiproteases hydrogels or combined with absorptionenhancers such as cyclodextrins bile salts and surfactants Insulin was also encapsulated in polymericbiodegradable nanocapsules nanoshperes or microparticles associated or not to surfactants orantiproteasas In this study nano particle were manufactured according to the double emulsion techniquemainly used for peptides and proteins Then many attempts have been conducted to make newformulation for orally insulin and decrease the consequence of insulin injection daily To improve thestability of insulin against gastrointestinal enzymes it was coated by using Edrugait polymer ThenNanopolymer coating insulin was tested in rabbit as animal model This Nano polymer decreased plasmaglucose level After testing Nano polymer on rabbit they were delivered orally to 15 sheeps for 5 dayperiod Blood samples were collected three times The concentration of cortisol estrogen insulin andIGF I were analyzed The results showed that concentration of cortisol were significantly in higher leveland progesterone in lower value Cortisol was 2 7 g dl and progestron was 2 5 ng ml In the secondexperiment IGF I was coated as Insulin did As above follow Nano polymer of IGF I decreased the levelof plasma glucose in rabbit In order to accessibility to duodenum of sheeps four sheeps were surgery andstudied for 5 day period Blood samples were collected on the first third and fifth day of period Theconcentration of progesterone and IGF I were significantly in higher level Progestron was 6 2 ng ml andIGF I was 69 5 ng ml Then we delivered Nanopolymer of IGF I orally to 15 sheeps in a 5 day period The concentration of cortisol estrogen insulin and IGF I were measured The concentration of cortisolincreased significantly and progesteron and cortisol decreased Cortisol was 2 8 g dl Stradiol was 0 28ng ml and progestron was 2 9 ng ml Key WordNano particle Nano Drug Insulin Delivery Insulin like growth factor Insulin nano particle IGF I nanoparticle

حميدرضا رحماني، محمد خوروش

محمد علي ادريس

غلامرضا قرباني، بدرالدين ابراهيم طباطبائي