6955

6497

كارشناسي ارشد

اصلاح نباتات

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1390

[دوازده]، 66ص.: مصور، جدول، نمودار

ص.ع. به فارسي و انگليسي

آقافخرميرلوحي، قدرت اله سعيدي

مهدي رحيم ملك

24/5/91

محمدرضا سبز عليان، سعيد انصاري

1396/09/18

كتابنامه

كشاورزي

مهندسي كشاورزي

ID6497

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Study of Genetic Diversity in Iranian Sesame Sesamum indicum L Genotypes using ISSR SSR Markers Safieh Nabi Afjedi snabi112@yahoo com Date of submission February 23 2012 Department of Agronomy and Plant Breeding Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisor 1 Mirlohi Ph D Professor Supervisor mirlohi@cc iut ac ir 2 Saeidi Ph D Professor Supervisor gsaeidi@cc iut ac ir AbstractSesame is one of the oldest cultivated plants by human being and due to its high quality aromatic seed oil has beennamed the Queen of the oily seeds Information on the degree of genetic variation can help improve this valuablecrop One of the main uses of molecular markers in plant breeding is their application in assessment of plant geneticdiversity In the last decades different molecular markers have been developed and used extensively in plant breedingprograms The main uses of the molecular markers are in the finger printing of varieties allele introgression and studyof genetic variation in many plants In this research genetic variation of 46 sesame genotypes collected from differentareas of Iran was assed using both Simple Sequence Repeat SSRs and Inter Simple Sequence Repeats ISSR primers Inter simple sequence repeats markers are widely used in genetic diversity studies because they need no priorDNA sequence information and their development costs are low From 20 used ISSR primers 16 producedpolymorphic bands In total 140 scorable bands with the size range of 200 to 2000 base pair were produced Twoprimers with 14 polymorphic bands produced the highest and one primer with 3 polymorphic bands produced thelowest number of scorable bands Primers 222 and b6 respectively had the most and the least polymorphisminformation content PIC Gene variety was 0 27 and Shanon information index was calculated to be 0 42 The meanof fragments for each primer in the studied genotypes was 7 87 Using NTSYS pc 2 02 with Jaccard similarity matrixand UPGMA method a dendrogram was drawn according to ISSR data of genotypes Base on this analysis thegenotypes were categorized in to 5 main groups In addition to ISSR primers 10 SSR primers were also used to studythe genetic variation of the 46 sesame genotypes Of the 10 pair of SSR primers used only 8 pairs created polymorphicPCR bands In total 30 bands allele were created and scored The number of alleles propagated by each of theseprimer pairs varied from 2 to 6 with an average of 4 alleles per locus The size of amplified fragments ranged from150 to 300bp The rate of heterozygosity polymorphism information content PIC and the gen diversity in thestudied genotypes were 66 51 and 58 respectively Using NTSYS pc 2 02 with Jaccard similarity matrix andUPGMA method a dendrogram was drawn according to SSR data of genotypes Base on this analysis the genotypeswere categorized in to 9 main groups Cluster analysis and Principal component analysis for two molecular markersystems showed that most genotypes were not grouped based on their geographical distribution Key words sesame genetic diversity microsatellite

آقافخرميرلوحي، قدرت اله سعيدي

مهدي رحيم ملك

محمدرضا سبز عليان، سعيد انصاري