به كارگيري پيوگليتازون در جيره نشخواركنندگان و اثر آن بر تخمير شكمبه، برخي از فراسنجه هاي خوني و مصرف خوراك

چكيده انگليسي :

Feeding of Pioglitazone in Ruminants and Its Effects on Ruminal Fermentation Some Blood Parameters and Dry Matter Intake Seyed Mehdi Ghoreishi ghoreishi@ag iut ac ir Hamid Reza Rahmani hrahmani@cc iut ac ir Masood Alikhani alikhani@cc iut ac ir Hamid Rajaian Mohammad Khorvash AbdoReza Hajipour Department of Animal Science Isfahan University of Technology Isfahan 84156 83111 IranAbstractIn the experiment1 voltametric determination of Piglitazone PGT as an alternative method wastested however this method does not respond In the experiment 2 in vitro digestion of PGT usingTilley and Terry method and effects of PGT on digestion of some feedstuffs via Ankom DaisyIncubatorII was evaluated The results showed that disappearance of 100 and 200 mg PGT was notsignificant after 48 h in vitro digestion In addition PGT has no adverse effects on in vitro digestionof some feedstuffs after 24 and 48 h incubation in Ankom Daisy IncubatorII These results indicatedthat unprotected PGT could be used in feeding of ruminants For determination of appropriated doseof PGT in ruminant bioavailability and some other pharmacokinetics parameters of PGT wereassessed in experiment 3 A single intravenous IV or oral dose of PGT 10 mg kg of body weight was administered to 5 male sheep Blood samples were collected at various time intervals and PGTconcentration was measured by a validated high performance liquid chromatography HPLC method The data obtained were best fitted into a two compartment model for the intravenous route and non compartmental approach for oral route The bioavailability of PGT was obtained to be about 62 After oral administration of PGT highest drug concentration observed in plasma Cmax the time tmax at which Cmax occurs and half life t1 2 were obtained to be 10 2 1 3 g mL 6 4 0 3 h and 4 42 0 21 h respectively These data showed that PGT has a marked post ruminal absorption which resultsin a noticeable bioavailability in sheep and apparently rumen microorganisms exert little degradationeffect on PGT In this study sheep used as a ruminant model and prior to feeding of PGT in dairycow in experiment 4 a single oral dose of PGT 10 mg kg was administered to 3 non pregnant drycows Blood samples were collected at various time intervals and PGT was detected by a validatedHPLC method In experiment 5 sixteen multiparous Holstein cows from 10 d before expectedparturition until 30 after parturition were allocated in treatment and control group and were fed ad libitum with same diet with and without PGT Pioglitazone 4 mg kg of body weight was topdressedfrom 10 till 20 relative to parturition after morning feeding Feeding of PGT increased dry matterintake DMI during the peripartum period d 5 to 5 P 0 05 however BCS body weight andnet energy balance were not affected by treatment There was no effect of feeding of PGT on milkyield and some milk composition for the first 30 d postpartum however 4 fat corrected milk milkfat and kg d and total solids were increased in cows were fed PGT Plasma non esterified fattyacid NEFA concentrations tended to be decreased during the peripartum period P 0 09 anddecreased during the postpartum period d 1 to 30 P 0 05 due to feeding of PGT but othermeasured blood parameters insulin glucose TG cholesterol and BHBA were not affected bytreatment Postpartum NEFA cholesterol ratio was higher in control group than treatment cows P 0 05 Effects of PGT administration on pregnancy duration calf birth weight and most plasmaparameters of calves were not significant P 0 05 however plasma TG concentration in calves oftreatment cows was lower than control group calves P 0 05 These results suggest that thepotential of PGT to increase periparturient DMI and decrease plasma NEFA concentration ofpostparturient dairy cows may improve metabolic health in dairy cow Keywords Pioglitazone Thiazoidinedione bioavailability transition cow

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به كارگيري پيوگليتازون در جيره نشخواركنندگان و اثر آن بر تخمير شكمبه، برخي از فراسنجه هاي خوني و مصرف خوراك

پيوگليتازون، تيازوليدين دايون، زيست فراهمي، گاوهاي دوره ي انتقال