ساختار جمعيتي عروس ماهي زاينده رود ﴿Petroleuciscus esfahani﴾ در رودخانه زاينده رود در پاسخ به آلودگي كادميم

چكيده انگليسي :

Population Structure of Zayandehrud Chub Petroleuciscus esfahani in Zayandehrud River in Response to Cadmium Pollution Neda shojaee neda shojaee2000@gmail com September 18 2012 Department of Natural Resources Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Dr Salar Dorafshan sdorafshan@cc iut ac ir AbstractPetroleuciscus esfahani a member of Cyprinidae family widely distributed in Zayandehrud and KaroonRiver basins in Iran Considering the importance of the genetic variation and population structure formanagement and conservation of species especially native fish the genetic diversity of Zayandehrud chubwas investigated using molecular techniques in response to Cadmium pollution In this study 30 specimens ofZayandehrud chub were collected from each locality Khersoonak KH Cheshmeh Dimeh CHD Safaeiehbridge S B and Chamgordan CH stations Cadmium concentration in gill muscle kidney and intestinetissus were determed with atomic absorption spectrometry For molecular study DNA was extracted usingBio basic kit of Genet Bio from bodies tissues collected from each stations The DNA quality was examinedby electrophoresis on 1 agaros gel and painted with ethidium bromide PCR amplification was performedusing five pairs of microsatellite primers CtoF 172 BL1 2b CnaB 030 LleA 071 and Ca3 at annealingtemperature of 56 58 C PCR products electrophoresed on 12 achrylamid gel and painted with silvernitrate PowerMarker ver 3 0 PopGene ver 3 2 ARLEQUIN ver 3 11 MEGA ver 4 and NTSYS ver 2 02softwares were using for data analysis The level of Cadmium in muscles was the lowest while in kidneytissue was the highest because of the aggregation and long preservation in tissue Pollution concentrationincrease from upstream KH and CHD to down stream S bridge and CH because of sewage of steel meelarrived to down stream Number of the observed alleles at all station ranged from 9 6 CHD to 8 6 3 otherpopulation and number of effective alleles was from 6 84 CHD to 5 66 CH The mean of polymorphisminformation content PIC at all loci was 0 826 Among the five pair markers CnaB 030 was the lesspolymorphic and Ca3 the most polymorphic one The expected heterozygosity ranged from 0 82 in KH CHand 0 85 in CHD and averaged 0 849 The observed heterozygosity ranged from 0 92 in CH and 1 00 in KHand averaged 0 978 Significant deviations from Hardy Weinberg Equilibrium expectation were found atCnaB 030 and Ctof 172 in all populations BL1 2b in KH CHD S bridge LleA 071 in KH S bridge andCa3 in CH population p 0001 The analysis of molecular variance AMOVA indicated that the percent ofvariance among populations and within individuals were 2 20 and 97 80 respectively Only a smallproportion 2 20 of the variation was attributed to differences between the populations and most of thevariation was attributed to differences within individuals The overall value of FST between populations was0 025 The genetic similarity and distance index came in the range of 0 756 0 859 and 0 141 0 244 respectively The level of genetic variability was high in the studied stations but comparison of meanexpected heterozygosity values revealed that these loci did not show significant differences between thestations There were existed significant differences in genetic variation among the stations and significantgenetic differences was found between pairwise of stations too p 005 that could be through Cadmiumpollution however existance of private alleles in all stations emphasis this subject but this differences couldnot separate stations from each other The low genetic differences between stations could be due to lowgeographic distance and high gene flow between stations For better understanding of population structureof Zayandehrud chub in response to pollutions population structure studies using other molecular techniquesand other pollutions from all distributed areas followed by ecological and or biological researches should beconsidered Keywords Zayandehrud chub Petro

شجاعي، ندا

ساختار جمعيتي عروس ماهي زاينده رود ﴿Petroleuciscus esfahani﴾ در رودخانه زاينده رود در پاسخ به آلودگي كادميم

فلز سنگين , ريز ماهواره , تنوع ژنتيكي , تفاوت ژنتيكي