پديد آورنده :
نوري، محبوبه السادات
عنوان :
اثر جداسازي ليگنين و همي سلولز بر بيو جذب آنزيمي تركيبات ليگنوسلولزي
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
مهندسي شيمي
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده مهندسي شيمي
صفحه شمار :
شانزده، 99ص.: مصور، جدول، نمودار
يادداشت :
ص.ع. به فارسي و انگليسي
استاد راهنما :
كيخسرو كريمي، اكرم زماني
توصيفگر ها :
اتانول , پيش فرآوري قليايي , چوب كاج , چوب نارون , دفع آنزيمي
تاريخ نمايه سازي :
27/9/91
استاد داور :
محمدرضا احساني، سعيد نوري خراساني
تاريخ ورود اطلاعات :
1396/09/22
رشته تحصيلي :
مهندسي شيمي
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
111 Effect of Lignin and Hemicellulose Separation on Enzymatic Bioadsorption of Lignocellulosic Compounds Mahboobeh Sadat Noori mnoori 672@gmail com 18 September 2012 Department of Chemical Engineering Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisor Keikhosro karimi karimi@cc iut ac ir Akram Zamani zamani akram@cc iut ac ir Abstract By growing the population over the world and the industrialization of countries the energy demand has been increased At the same time shortage and depletion of oil resources has increased attentions towards bioethanol production as a clean and renewable energy resources Lignocellulosic materials are one of the renewable and low cost resources which are abundantly available In this study after a physical treatment chopping on pine and elm woods an alkaline pretreatment was performed with 8 w v NaOH at three different temperatures 0 25 and 80 C for 2 h to improve the yields of enzymatic hydrolysis and ethanol production processes Alkaline pretreatment by increasing accessible surface area for hydrolytic enzymes and decreasing the cellulose crystallinity and degree of acetylation of hemicelluloses and also delignification can modify the structure of lignocellulosic materials To study the effect of the pretreatment enzymatic hydrolysis was conducted on the untreated and pretreated woods at 45 C for 72 h with 30 FPU cellulase and 60 IU glucosidase per grams of substrates Furthermore simultaneous saccharification and fermentation SSF for production of ethanol was done at 36 C for 72 h using 15 FPU and 30 IU per grams of substrates The structural properties of the materials were followed by SEM images and FTIR analysis The results showed decreasing structural uniformity and crystallinity For both kinds of wood maximum increase in water adsorption capacity was observed for the pretreated samples at 0 C On the other hand alkaline pretreatment had a significantly improved the buffering capacity of the woods Furthermore enzyme deactivation that occurred as a result of ineffective adsorption of enzymes on lignin was investigated by adsorption desorption analysis However no logical relation obtained between adsorbed and desorbed enzymes and enzymatic hydrolysis results In order to decrease the ineffective adsorption of enzyme on lignin a non ionic surfactant Tween 20 was added to the mixture of enzymes and substrates Results of hydrolysis showed that the maximum yield for pine and elm woods pretreated at 0 C improved to 18 7 and 71 5 respectively Addition of tween 20 increased the maximum enzymatic hydrolysis yields of pretreated pine and elm to 19 6 and 80 1 respectively Yield of ethanol production in SSF step for untreated elm wood was 8 4 while it was increased to 45 8 after pretreatment at 0 C For the same pretreatment on pine wood the yield was increased from 8 to 16 4 Using tween 20 increased ethanol production yield of pine and elm woods to 23 9 and 57 3 respectively In the presence of tween 20 maximum amount of enzyme adsorption was 49 6 mg per gram of pine wood while the maximum amount of desorption was 44 1 Key Words Alkali pretreatment Elm wood enzymatic adsorption enzymatic desorption ethanol hemicellulose lignin Pine wood
استاد راهنما :
كيخسرو كريمي، اكرم زماني
استاد داور :
محمدرضا احساني، سعيد نوري خراساني
