پديد آورنده :
هدايي، محبوبه سادات
عنوان :
بررسي تنوع سيتوپلاسمي لاينهاي آلوپلاسم گندم با استفاده از نشانگرهاي ريز ماهواره كلروپلاستي
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
اصلاح نباتات
محل تحصيل :
اصفهان : دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
يازده، 53ص.: مصور، جدول، نمودار
يادداشت :
ص.ع. به فارسي و انگليسي
استاد راهنما :
احمد ارزاني، مهدي رحيم ملك
تاريخ نمايه سازي :
7/11/91
استاد داور :
آقافخر ميرلوحي، محمدعلي ادريس
تاريخ ورود اطلاعات :
1396/09/22
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Assessment of Cytoplasmic Variation in Wheat Alloplasmic Lines Using Chloroplastic Microsatellite Markers Mahboobeh Hodaei@yahoo com Department of Agronomy and Plant Breeding Isfahan University of Technology Isfahan 84156 83111 Iran Degreee M Sc Language Farsi Abstract Exploitation of wheat germplasm requires the knowledge of genetic diversity of the plant materials Although inheritance of plant traits in plants mainly depends upon nuclear genes cytoplasmic factors and their interactions also play important role In this regard the development and utilization of alloplasmic lines have facilitates the cytoplasm genetic studies Due to the slow evolution of the chloroplastic genes chloroplastic microsatellite loci whose evolutionary speed is several times higher than other regions of the chloroplast genome are suitable candidate for analysis of cytoplasmic variation Therefore 32 alloplasmic lines accompanied with a euplasmic parental line control were evaluated using morph physiological traits and molecular markers microsatelite during 2010 2011 and 2011 2012 using randomized complete block design The results showed that 20 primer pairs out of 26 chloroplastic microsatellite markers cpSSR are polymorphic A total of 50 alleles were identified with an average of 2 5 alleles per locus In this study mean of polymorphism information content PIC was 0 33 and ranged from 0 05 in WCt17 primer to 0 49 In WCt9 primer After scoring electrophoresis bands from each primer pair using software Mega4 distance matrix based on p distance was calculated to compare the two group of alloplasmic lines Genetic distance between alloplasmic lines ranged from 0 to 0 76 with an average of 0 28 Cluster analysis of molecular data revealed that used alloplasmic wheat lines belongs to two major clusters Hence differentiation of the cytoplasmic type and of the genus Triticum و Aegilops has been evident Analysis of molecular variance AMOVA also showed significant differences between the two study groups FST 0 67 P 0 001 Based on the analysis morpho physical data cytoplasm had no significant influence on chlorophyll content in leaves dry weight number of grain per spike flag leaf length peduncle length and spike length On the other hand height photosynthesis and seed yield has been significantly affected by cytoplasm It is concluded that hexaploid and tetraploid derived cytoplasms performed superior than diploid cytoplasm in wheat However The dendrogram obtained from morphological data failed to fully segregate the two genera Results comparing the similarity of two clusters of molecular and morphological data showed no significant relationship This inconsistency can be justified based on hypothesis that amplification of cpSSR markers specific for the repetitive regions of the chloroplast genome within non coding regions while the coding regions of the genome is expressed via morphological markers Overall the results showed that the primers used can be valuable resources of polymorphic markers for the analysis of cytoplasm of Triticeae species with the potential of clearly differentiate in close species and genera of this tribe key words Wheat Alloplasm Cytoplasmic type Chloroplast Microsatellite photosynthesis yield
استاد راهنما :
احمد ارزاني، مهدي رحيم ملك
استاد داور :
آقافخر ميرلوحي، محمدعلي ادريس
