7595

7067

كارشناسي ارشد

علوم باغباني

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1391

ده،61ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

سيروس قبادي

بهرام باني نسب

17/1/92

علي اكبر رامين، مجيد طالبي

مهندسي كشاورزي

ID7067

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

In vitro Propagation and Shoot Proliferation of two June and Everbearing Strawberry Cultivars Utilizing Cut achene and Embryo culture Parvin Daliranzadeh p daliranzadeh@yahoo com 6 January 2013 Department of Horticultural Science Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisor Assist Prof C Ghobadi Cyrus@cc iut ac ir Abstract Strawberries as one of the small fruits of temperate regions have been a popular delicious fruit for its flavor taste fresh use and become the most important and commercial horticultural produces in recent decades Strawberry cultivars are highly heterozygote so every seedling in the population is genetically unique Thus the use of achenes is important as a true representative of the plant populations in order to produce seedlings Since germination of seeds of strawberry cultivars is not uniform achieving an efficient techniques for uniform seedling production in short term along with increasing the branch crown numbers are important for the mass production In this study in separate experiments germinability of stratified and Non stratified achenes of two strawberry cultivars Fragaria x ananassa Duch Selva and Camarosa by using the cut achenes and embryos as explants and utilizing Prohexadione Calcium Pro Ca in various concentrations 0 5 1 5 3 4 5 6 mM in order to enhance branch crowns were examined in vitro in factorial experiments in completely randomized design with four replications the excised embryos and the cut achene were cultured in MS basal medium lacking hormones the cultures were stored up to 8 weeks at 27 C The results indicated that the maximum germination percentage 90 100 germination rate two three days and the rate of normal seedling regeneration 80 90 percent in explants of cut achenes and embryo culture comparing to whole seeds were high and it was clearly observed in two cultivars Utilizing Prohexadione Calcium at the concentrations of 1 5 4 5 and 6 mM obviously increased crown branching in Camarosa cultivar and no effect on Selva cultivar The number of induced branch crowns in Camarosa cultivar at 4 5 and 6 mM was twice more than of 1 5 mM of applied Pro Ca Exogenously applied Pro Ca had significant effect on developmental indices and decreased the main stem length main stem diameter and enhanced number of leaves and roots and wet and dry weight of regenerated seedlings In conclusion the main results of this study was confirmed that in vitro application of Pro Ca as an anti GA chemical has the potential to differentiate and develop the branch crowns in strawberry that is genotype dependent absence of physiological dormancy in strawberry seeds and identifying the hard seed coats as a preventing agent of uniform seed germination Keywords Strawberry Crown branch Prohexadione Calcium Cut achene Embryo culture

سيروس قبادي

بهرام باني نسب

علي اكبر رامين، مجيد طالبي