7702

7174

كارشناسي ارشد

شيمي معدني

اصفهان: دانشگاه صنعتي اصفهان، دانشكده شيمي

1391

چهارده،89ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

حسن حدادزاده، رحمان جهانيان

شادپور ملك پور

17/2/92

كاظم كرمي، حسن قاضي عسگر

شيمي

ID7174

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

The amino acids complexes of Zn II structure spectroscopic properties and their inhibitory effects on the growth of Aspergillus Flavus and production of Aflatoxin B1 Nafiseh Jarrah n jarrah@ch iut ac ir Date of Submission 2013 1 14 Department of Chemistry Isfahan University of Technology Isfahan 84156 83111 Iran Degree M S Language Farsi H Hadadzadeh Prof hadad@cc iut ac ir R Jahanian Assist Prof r jahanian@cc iut ac ir Abstract In this thesis the complexes of Zn II with amino acid ligands Zn Gly 2 Zn Gln 2 Zn His 2 Zn Arg 2 OAc OAc 3H2O Zn Met 2 and Zn Cys 2 where Gly Glycine Gln Glutamine His Histidine Arg Arginine Met Methionine and Cys Cysteine have been prepared and characterized by elemental analysis FT IR and UV Vis spectroscopies The solid state structure of Zn Arg 2 OAc OAc 3H2O and Zn Gln 2 complexes were determined by single crystal X ray crystallography The colourless crystals of Zn Arg 2 OAc OAc 3H2O were grown by slow evaporation of propanol water solution of the complex The complex was crystallized in monoclinic crystal system with P21 space group Single crystal structure showed that the coordination geometry around Zn II was distorted square pyramidal The basal positions of the pyramid have been occupied by two Arg ligands as bidentate chelate with a similar configuration One acetate ligand has been coordinated in apical position of pyramid via an oxygen atom The crystals of Zn Gln 2 were grown by ethanol diffusion into an aqueous solution of the complex Single crystal structure showed that the coordination geometry around the Zn II was a distorted octahedron The Gln ligands are coordinated to Zn II ions through the carboxylate and amine groups in the equatorial positions Two axial positions are occupied by neighbouring Gln molecules via the carbonyl oxygen atom therefore this complex has a polymeric structure The FT IR spectra of the ligands and the complexes showed that the carboxylate and amine bands of the amino acid ligands are shifted to higher frequencies Electronic spectra of these complexes were taken in water The effect of the complexes as inhibitors of fungal growth and aflatoxin production by Aspergillus flavus was investigated in six periods of biological experiments In each set of experiments the solutions with different concentrations of water soluble complexes were added to PDA medium containing spores of Aspergillus flavus The culture mediums were placed in the incubator for 6 days at 25 30 C The amount of aflatoxin produced in vitro in the presence of different concentrations of these complexes was changed significantly Generally with increasing concentration of each of the complexes there was almost no growth of the fungus Aspergillus flavus and the amount of aflatoxin produced was minimal The toxin produced by the fungus in culture medium was measured using ELISA Key word Zn II complexes Amino acids Crystal structure Aspergillus Flavus Aflatoxin

حسن حدادزاده، رحمان جهانيان

شادپور ملك پور

كاظم كرمي، حسن قاضي عسگر