بررسي تنوع ژنتيكي نسل هاي F5 و F6 و جمعيت هاي حاصل از انتخابات در تلاقي هاي بين گونه اي گلرنگ اهلي-گلرنگ وحشي

چكيده انگليسي :

Evaluation of genetic variation in F5 and F6 generations and populations derived from interspecific hybridization between wild and cultivated safflower Somaye Senobari s senobari@yahoo com Date of submission 23 January 2013 Department of Agronomy and Plant Breeding Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisor G Saeidi Saeidi @ cc iut ac ir Abstract Safflower is an important oilseed crop and is cultivated in different countries around the world Using the genetically improved cultivars of the crop can cause higher seed yield production and oil quality The main requirement for plant breeding is the exiting of genetic variation in the populations of the plant species and because of its limitation in cultivated safflower germplasm enhancement of genetic variation in this plant species can be helpful in breeding programs In this study genetic variation of agronomic traits in populations derived from interspecific hybridization and also genetic variation in breeding lines isolated from these populations were investigated in two separate experiments In the first experiment planting date March 2012 four populations derived from interspecific hybridization between C tinctorius C oxyacanthus at generation F5 and four populations from this crosses at generation F6 and 3 genotypes of the parental lines along with three control cultivars totally 14 entries evaluated in a randomized complete block design with three replications In this experiment single plant selection was performed based on agro morphological traits to isolate some breeding lines In the second experiment planting date March 2013 the isolated breeding lines along with the parental genotypes and Ac Stirling cultivar 121 entries were evaluated in a simple lattice design with two replications The results of first experiment showed that there were significant differences among the genotypes for all studied characters except for number of branches per plant The results of analysis of variance for the second experiment also showed that the isolated lines were significantly different for all studied traits The characters of heads per plant seeds per heads grain yield per plant and harvest index had the highest phenotypic and genotypic coefficients of variation in both experiments In the first experiment there was no significant difference between genotypes of generation F5 vs F6 derived from interspecific hybridization for all the traits except the biological yield In the first experiment 1000 grain weight harvest index and head diameter had the greatest genetic and phenotypic correlation with grain yield per plan The results of stepwise regression in the first experiment also indicated that 1000 grain weight head diameter and number of heads per plant were the most important components of seed yield per plant These results indicate that these traits can be used as selection criteria for improving grain yield in breeding programs The path coefficient analysis in the first experiment also showed that among seed yield components 1000 grain weight had the greatest positive direct effect on seed yield per plant Cluster analysis indicated that the genotypes of the first experiment were classsified in two groups of 5 and 9 genotypes However the genotypes were classified in three groups of 38 22 and 61 in the second experiment In both experiments the wild and cultivated genotypes were classified in two distinct groups Principal components analysis showed that the first and second main components explained 82 and 64 of the total variation respectively also this analysis classified the genotypes to three separate groups These results showed that here was a high agreement between the pattern of distribution for the genotypes in principal component and cluster analysis In the second experiment the breeding lines of 108 106 121 and 71 had the highest seed yield per plant and were not significantly different from the high yielding cultivate

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بررسي تنوع ژنتيكي نسل هاي F5 و F6 و جمعيت هاي حاصل از انتخابات در تلاقي هاي بين گونه اي گلرنگ اهلي-گلرنگ وحشي

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