7795

7266

كارشناسي ارشد

بيماري شناسي گياهي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1391

ده،52ص.: مصور،جدول،نمودار

ص.ع.به فارسي و اگليسي

امير مساح، بهرام شريف نبي

29/3/92

مسعود بهار، بدرالدين سيد ابراهيم طباطبائي

مهندسي كشاورزي

ID7266

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Comparison of certain Iranian maize mosaic virus isolates using nucleocapsid protein gene sequencing Samira Kheirabadi kh sa65@yahoo com Date of Submission January 22 2013 Department of Plant Protection Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi A Massah Ph D Assist Professor Supervisor amassah@cc iut ac ir B Sharifnabi Ph D Professor Supervisor Sharifna@cc iut ac ir Abstract Corn along with wheat and rice is one of the most important cereal crops in the world hence its cultivation and production as a strategic crop being increased in the world and Iran Iranian maize mosaic virus IMMV a member of Nucleorhabdovirus genus and Rhabdoviridae family was found in a corn field in Fars province in 1979 The virus host range is restricted to the family Gramineae and is one of the most destructive viruses of maize in Iran IMMV infected plants exhibit chlorotic stripes paralell to the main midrib in all or a part of the leaf Although the almost complete nucleotide sequence of IMMV Shiraz isolate is available at GenBank however there is no information on molecular variation of IMMV isolates in Iran In this study in order to detection and molecular comparison of IMMV isolates in fall 2011 and spring and summer 2012 leaf corn and wheat samples with viral symptoms were collected in Isfahan Chaharmahal o Bakhtiari Lorestan and Fars provinces Total RNA was extracted from leaf samples and used as template in RT PCR with AMLF AMLR primer pair amlifying a part of the polymerase gene of IMMV Amplification of expected size of 720 bp segment confirmed infection of all corn samples and some wheat samples from Isfahan province 15 out of 70 to IMMV This is the first report of wheat infection to IMMV in Isfahan province For molecular comparisons full length of IMMV nucleoccapsid protein gene 1480 bp was amplified in RT PCR using AMNupNHE AMNloEcoRl primer pair in infected samples Seven isolates representing different hosts and regions including Farrokhshahr FZ2 Kouhdasht ZK2 Daran ZA9 Dastgerd Bk1 and Shiraz OZ1 isolates from maize and Joharestan Gg2 and Ziar GZ5 isolates from wheat were sequenced Multiple allignment of nucleocapsid protein gene of our isolates and one isolate of IMMV DQ186554 available at GenBank in nucleotide level revealed high identity ranging from 98 5 to 99 8 among isolates Farrokhshahr isolate FZ2 showed the lowest identity with other isolates This isolate showed the lowest 98 5 and highest identity 98 9 with Bk1 isolate and ZK2 and ZA9 isolates respectively The ZK2 isolate showed the highest identity 99 8 with OZ1 isolate and the lowest identity 98 9 with FZ2 isolate In phylogenetic analysis the isolates were divided into two subgroups however no correlation was found between hosts and geographical regions Key word Iranian maize mosaic virus Nucleocapsid protein gene Phylogenetic tree

امير مساح، بهرام شريف نبي

مسعود بهار، بدرالدين سيد ابراهيم طباطبائي