8140

7538

كارشناسي ارشد

بوم شناسي آبزيان شيلاتي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده منابع طبيعي

1391

دوازده،71ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

سالار درافشان، فاطمه پيكان حيرتي

نصراله محبوبي صوفياني

20/8/92

يزدان كيواني، مجيد طالبي

مهندسي منابع طبيعي

ID7538

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Evaluation of some biological characteristics of Petroleuciscus esfahani in response to endocrine disrupting chemicals EDCs in the Zayande Roud River Neda Gilannejad Email address neda guilannejad@gmail com Date of submission Department of Natural Resources Isfahan University of Technology Isfahan Iran Degree M Sc Language Farsi Supervisors Dr Salar Dorafshan sdorafshan@cc iut ac ir and Dr Fatemeh Paykan Heytrati fheyrati@cc iut ac ir Abstract Endocrine disruptor compounds EDCs are a type of environmental pollutants with both natural and anthropogenic origins Final destination for most of these pollutants is aquatic environments Therefore they can affect aquatic wildlife species through interfering with their endocrine system and causing detrimental effects on them The aim of this study was the evaluation of some biological characteristics such as hematological factors sex ratio gonado somatic index GSI and vitellogenin VTG expression in Petroleuciscus esfahani caught from Zayandeh Roud River A total of individuals were collected from four sampling stations Cheshmeh Dimeh CHD and Khersoonak KH located upstream the river and Chamgordan CH and Safaeieh Bridge SB from downstream the river Averaged length and weight for the specimens from the four stations were g and mm respectively Evaluation of hematological factors RBC Hb HCT and WBC showed no significant differences among stations But the average percentages of all leukocyte types were significantly higher in CH except for lymphocytes p On the other hand MCV MCH and MCHC were significantly lower in CH in comparison to the other sampling stations p In all the stations sex ratio showed deviations from the expected ratio in favor of females except for SB Chi square test p Microscopic assessment of the gonads did not show any type of disorder such as intersex GSI was compared in males and females from all the stations The only statistically significant difference was observed in male GSI from CH which was lower than the male GSI from the other stations p VTG expression was used as a reliable marker for exposure of fish to EDCs with estrogenic properties For this purpose it was necessary to clone VTG cDNA for the first time in Petroleuciscus esfahani Results indicated that the full length VTG cDNA consists of bp and with its respective putative protein having amino acids Evaluation of VTG expression with real time PCR using actin as the reference gene showed relative gene expression in both sexes from all four stations although it was significantly higher in males and females from CH p In general the data suggest that Petroleuciscus esfahani fish populations are exposed to EDCs with estrogenic activity which has affected some of its biological features such as sex ratio deviation toward females and vitellogenin gene expression in males as well as altering its expression pattern in the females caught from Zayandeh Roud River Keywords Endocrine disrupting compounds EDCs Gonad histology Haematology Petroleuciscus esfahani Vitellogenin expression PDF created with pdfFactory trial version www pdffactory com

سالار درافشان، فاطمه پيكان حيرتي

نصراله محبوبي صوفياني

يزدان كيواني، مجيد طالبي