8144

7542

كارشناسي ارشد

علوم باغباني﴿گياهان زينتي﴾

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1392

دوازده،62ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

اعتمادي، طالبي

علي نيكبخت، بدرالدين ابراهيم سيد طباطبائي

25/8/92

مهديه غلامي، محمد مهدي مجيدي

مهندسي كشاورزي

ID7542

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Evaluation of genetic diversity of plane Platanus orientalis trees by morphological characteristics and SRAP molecular markers Milad Orojloo m orojlou@ag iut ac ir Date of submission Jun Department of Agricultural Horticulture Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi N Etemadi Assoc Professor Supervisor Etemadin@cc iut ac ir M Talebi Assist Professor Supervisor mtalebi@cc iut ac ir Abstract Landscape is more importance especially in urban areas Among broad leaved trees the plane Platanus sp has major role in establishing landscape areas This tree belongs to Platanaceae family The geographical distribution of this genus includes from east mideterranean regions to northern America and Groenlands Among Platanus species it is expected that only the P orientalis would be native to Iran With respect to plane importance in urban landscapes prior to found and select elite genotypes or varieties the evaluation of possible differences in according to morphological and genetic characteristics among plane trees of various regions is necessary For evaluation of genetic diversity among and within plant populations morphological studies are one of the useful methods In this respect for evaluation of parameters such as number of lobes per leaf leaf shape succulent rate number of fruit and dropping pattern of bark the samples of P orientalis from Isfahan Arak Karaj Mahallat Kashan and Natanz and also tow samples of P occidentalis were collected Among and within species relationships of studied planes were drawn by using data those were obtained from morphological characteristics For this purpose Jaccard s similarity coefficient and UPGMA algoritm with cophenetic correlation coefficient of has been used that indicating suitability of UPGMA method for sample classification The Results showed parameters there was separation ability between species but the intra specific difference was not suffiction to completely separate them Since the morphological characteristics could be affected by environmental conditions therefore using molecular methods to detect and classify species and varieties are of very important For determining genetic diversity among and within species SRAP marker that targets coding regions were used Thirteen used primer combinations on plane genotypes ampilified fragments that of them showed polymorphism Calculated average PIC value was for all primer combinations Cluster analysis with usage of Jaccard s similarity coefficient and UPGMA algorithm with cophenetic correlation coefficient of has been done Cluster analysis separated P orientalis and P occidentalis species well but samples of P orientalis located on one branch PCA analysis also confirmed results of cluster analysis Also Fst in this study was zero that indicates no genetic diversity within species It is seems that the propagation type of this plant that is cutting caused high genetic similarities within this species Keywords Plane genetic diversity morphological characteristics SRAP marker IranPDF created with pdfFactory trial version www pdffactory com

اعتمادي، طالبي

علي نيكبخت، بدرالدين ابراهيم سيد طباطبائي

مهديه غلامي، محمد مهدي مجيدي