8222

7620

كارشناسي ارشد

اصلاح نباتات

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1392

يازده،72ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

محمد مهدي مجيدي، آقافخرميرلوحي

16/9/92

محمدحسين اهتمام، مجيد طالبي

مهندسي كشاورزي

ID7620

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

73 Study of Genome Similarity and Genetic Variation of Some Brassica Species Using Morphological and Inter Simple Sequence Repeat ISSR Markers Mahshid pirboveiry m p boveiry@gmail com 21 september 2013 Department of Agronomy and plant Breeding Isfahan University of Technology Isfahan 84156 83111 IranDegree M s c Language FarsiSupervisors Majidi@cc iut ac irM Majidi Assoc Prof Mirlohi@cc iut ac irA Mirlohi Prof AbstractCanola rapeseed is one of the oilseed plants which is superior to other cultivated oilseeds in Iran due toappropriate and positive agronomical traits Canola is cold tolerant relatively tolerant to water limitedsituations better tolerant of saline conditions and has high rotation value It also has both spring and wintergenotypes and eventually higher oil yield in unit area Information on genetic diversity of crops has a greatdeal of importance in order to conserve genetic resources and germplasm to develop genetic background andalso its application in breeding programs Hence Brassica wild relatives have desirable and suitableagronomical traits such as cytoplasm and nuclear male sterility resistance against diseases insects andnematodes tolerance to cold salinity and drought stresses they can be exploited in breeding programs Toinvestigate the intra and inter species genetic diversity of Brassica based on morphological characteristic andISSR markers two experiments were conducted during 2012 2013 In the first study genetic diversitybetween 36 accessions of six Brassica species was evaluated for morphological traits using multivariatestatistical analysis The results indicated considerable variation in the studied germplasm for all traits Clusteranalysis based on morphological traits could separate genotypes of different species from each other In thesecond study genetic diversity between 56 accessions belonging to ten species was evaluated using ISSRtechnique From the 23 primers used 186 polymorphic markers were scored to differentiate betweenaccessions Accordingly ISSR analysis revealed that cultivated B napus accessions were less diverse thanother species Applying UPGMA method of cluster analysis could separate species appropriately from eachother Principal coordinate analysis PCoA confirmed the result of cluster analysis Results of molecularvariance analysis AMOVA based on species showed that 35 46 of diversity was related to betweenspecies and the remnant was attributed to diversity within species Results obtained from present studypointed out a low correlation 0 05 between clustered diagrams created from morphological data and usingISSR markers data Nevertheless using ISSR marker in comparison with morphological data geneticsimilarity between studied accessions was much better determined implying the ISSR marker efficiency indistinguishing species of Brassica genus Keywords Brassica morphology ISSR molecular cluster analysis phylogeny geneticsimilarity

محمد مهدي مجيدي، آقافخرميرلوحي

محمدحسين اهتمام، مجيد طالبي