8449

7830

كارشناسي ارشد

اصلاح نباتات

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1392

دوازده،62ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

مهدي رحيم ملك

حسين زينلي

5/11/92

محمدرضا سبزعليان، بهرام شريف نبي

مهندسي كشاورزي

ID7830

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

52 Genetic diversity of German chamomile Matricaria chamomilla using ISSR and morphological markers Hossein Ahmadi hosssein ahmadi1365@gmail com Submitted on 2013 Department of Agronomy and Plant Breeding Isfahan University of Technology Isfahan 84156 83111 Iran Degree M ScLanguage FarsiM Rahimmalek Assist Prof supervisor mrahimmalek@cc iut ac irAbstract Iran has many valuable genetic resources of medicinal plants It is important to exploit a variety of genes fromthis variation Considering the importance and various uses of medicinal plants today many aspects of plant scienceresearches have focused on the use of these plants German chamomile Matricaria chamomilla is an herbaceousplant perennial native to the Mediterranean region and Asteraceae family It is native to Mediterranean regions andsome researchers reported Asia as its origin This study evaluated the genetic diversity of 21 Iranian and twoEuropean populations using ISSR molecular markers and morphological characteristics According to the results out of 20 ISSR primers 10 one generated polymorphic bands with clear banding patterns A total of 208 bands weregenerated in which 193 were polymorphic bands 92 78 of the variation Bands were scored as zero for absenceand one for presence The genetic similarity coefficient was calculated based on Simple Matching SM 0 92 Results of cluster analysis and principal coordinate analysis PCoA of the chamomiles were divided samples intofour groups Analysis of molecular variance AMOVA showed more variation within groups 58 98 comparedwith the variation among groups 41 02 Based on the collection sites three groups were assumed for AMOVAanalysis including Centre C North West NW and South East SE Most of the genetic diversity was obtainedfor Center 0 25 and the lowest one was observed in South East 0 054 respectively Cluster analysis based onmorphological characters divided genotypes into two major groups Totally there were no correlation betweenmorphological and molecular results Finally Acceptable variation was observed in molecular and morphologicalresults for chamomiles and it is beneficial for future breeding programs Keywords Matricaria chamomilla Molecular marker Morphology

مهدي رحيم ملك

حسين زينلي

محمدرضا سبزعليان، بهرام شريف نبي