ارزيابي ژرم پلاسم نعناي ايراني در مقاومت به تنش هاي سرما و يخ زدگي

چكيده انگليسي :

Evaluation of Iranian Mint Germplasm for Resistance to Cold and Freezing Stresses Sepideh Rafatrad srafatrad@yahoo com June 19 2012 Department of Agronomy and Plant Breeding College of Agriculture Isfahan University of Technology Isfahan 84156 83111 IRAN Degree M Sc Language Farsi Supervisor M R Sabzalian Assist Prof sabzalian@cc iut ac ir M Rahimmalek Assist Prof rahimmalek@cc iut ac ir ABSTRACT Mint can be grown in the most parts of Iran but cold and very cold areas are not suitable for the cultivation of this plant The desired temperature for growth is 10 C So one of the factors limiting the plant growth in cold climates is low resistance to low temperatures Two experiments were conducted inthe field control and greenhouse incubator Greenhouse experiment was performed in a completely randomized designwith three replications In the stage 8 mint genotype including Tabas Qazvin Isfahan and Kermanshah from Mentha pipertia L Isfahan and Hamedan from Mentha longifolia L and Lorestan and ahvazfrom Mentha spicata L were evaluated at five temperatures of 10 5 0 5 10 C inside incubator In the field experiment eight genotypeswere planted in a randomized complete block design with three replications andwere evaluated under field temperature In incubator electrolyte leakage chlorophyll fluorescence leaf damage leaf area and proline content on the resistant and sensitive genotypes were measured In field experiment electrolyte leakage chlorophyll fluorescence leaf area essential oil percentage and spring growth were measured The results in controlled conditions electrolyte leakage chlorophyll fluorescence and symptoms of chilling injury showed that temperatures above zero 10 5 and zero have not significantly affected the genotype and damages have caused under freezing temperatures frost Assessment of chlorophyll fluorescence in controlled conditions incubator showed that none of the eight genotypes of mint were affected at temperatures above zero 10 5 and zero and the maximum and minimum of injury happened at 5 and 10 frost were belong to lorestan and hamedan respectively Comparing genotypes of mint regarding chlorophyll fluorescence and electrolyte leakage showed similarity in the results so thatahvazand Isfahan were the most resistant and sensitive genotype respectively based on fluorescence in both conditions and tabaswas the most resistant genotype based on electrolyte leakage In terms of electrolyte leakage under controlled conditions incubator the maximum leakage in freezing temperatures of 5 and 10 was observed in ahvaz The genotypes of lorestan and ahvazhad the maximum leaf areaand chlorophylle florescence in the field and control condition at 10 C The genotypes of isfahanand tabas from M piperita had the highest and lowest content of essential oil respectively after cold period The same genotypes had the most and the least spring growth respectively When temperature decreased the amount of proline in genotypes of pipertia species Tabas Qazvin Kermanshah and Isfahan increased Besides comparing these genotypesshowed that the genotype Isfahan had the minimum content of proline in response to stress conditions Keywords Cold stress freezing stress mint germplasm resistance PDF created with pdfFactory trial version www pdffactory com

رفعت راد، سپيده

ارزيابي ژرم پلاسم نعناي ايراني در مقاومت به تنش هاي سرما و يخ زدگي