پديد آورنده :
نعيمي سرشت، ميترا
عنوان :
بررسي تمايز سلول هاي بنيادي مزانشيمي كندروسيت در داربست نانو كامپوزيتي برپايه ابريشم حاوي نانو ذرات كيتوسان
گرايش تحصيلي :
مواد- بيومواد
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده مواد
صفحه شمار :
چهارده،108ص.:مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
محمدحسين فتحي،محمد رفيعي نيا
استاد مشاور :
شاهين بنكدار
توصيفگر ها :
مهندسي بافت , بيومواد , فيبروئين , غضروف
تاريخ نمايه سازي :
10/9/93
استاد داور :
شقايق حق جو، عباس تيموري،حسين توانايي
كد ايرانداك :
ID685 دكتري
چكيده انگليسي :
119 Evaluation of Differentiation of Mesenchymal Stem Cells into Chondrocyte on Silk based Scaffolds Containing Chitosan Nanoparticles Mitra Naeimi Seresht m naeimi@ma iut ac ir Date of Submission Nov 1 2014 Department of Materials EngineeringIsfahan University of Technology Isfahan 8415683111 Iran1st Supervisor Mohammadhossein Fathi Professor fathi@cc iut ac ir2nd Supervisor Mohammad Rafienia Associate Professor m rafienia@med mui ac irAbstractThe development of porous biodegradable scaffolds is of great interest in tissueengineering In this regard exploration of novel biocompatible materials is needed Silkfibroin chondroitin sulfate sodium alginate SF CHS SA porous hybrid scaffolds weresuccessfully prepared via lyophilization method and crosslinked by 1 Ethyl 3 3 dimethylaminopropyl carbodiimide EDC ethanol treatment According to the scanningelectron microscopy studies mean pore diameters of the scaffolds were in the range of 60 187 m The porosity percentage of the scaffold with SF CHS SA ratio of 70 15 15 w w w was 92 4 3 Attenuated total reflectance Fourier transform infraredspectroscopy X ray diffraction and differential scanning calorimetry results confirmed thetransition from amorphous random coil to crystalline sheet in treated SF CHS SAscaffold Compressive modulus was significantly improved in hybrid scaffold with SF CHS SA ratio of 70 15 15 3 35 0 15 MPa Cytotoxicity assay showed that the scaffoldshave no toxic effects on chondrocytes Attachment of chondrocytes was much moreimproved within the SF CHS SA hybrid scaffold Real time polymerase chain reactionanalyses showed a significant increase in gene expression of collagen type II aggrecan and SOX9 and decrease in gene expression of collagen type I for SF CHS SA compared toSF scaffold This novel hybrid scaffold can be a good candidate to be utilized as anefficient scaffold for cartilage tissue engineering Keywords tissue engineering biomaterials stem cells silk fibroin cartilage1 IntroductionThe cocoon of Bombyx mori silkworm is mainly composed of sericin and fibroin 1 2 The silk fibroin SF fibers are consisting of two kinds of proteins a light chain 26 kDa and a heavy chain 390 kDa which are linked by a single disulfide bond These proteinsare coated with sericin a family of hydrophilic proteins 20 310 kDa The main aminoacids present in the silk fibroin from Bombyx mori are glycine Gly 43 alanine Ala 30 and serine Ser 12 3 Silk fibroin is an attractive material for biomedical
استاد راهنما :
محمدحسين فتحي،محمد رفيعي نيا
استاد مشاور :
شاهين بنكدار
استاد داور :
شقايق حق جو، عباس تيموري،حسين توانايي
