9618

8873

كارشناسي ارشد

شيمي تجزيه

اصفهان: دانشگاه صنعتي اصفهان، دانشكده شيمي

1393

[چهارده]،66ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

علي اصغر انصافي

بهزاد رضايي

93/12/17

محمد سراجي، محمدتقي جعفري

شيمي

ID8873

Isfahan University of Technology Department of Chemistry M Sc Thesis Biosensor based ds DNA for detection of DNA damage induced by Fe III and Cu II in the presence of dopamine and influence of some antioxidant on DNA damage By Narges Kazemnadi Supervisor prof Ali A Ensafi Department of Chemistry Isfahan University of Technology Isfahan 84156 83111 Iran Abstract There is increasing evidence supporting a causal role of oxidatively damaged DNA in neurodegeneration during the natural aging process and neurodegenerative diseases such as Parkinson s and Alzheimer s The presence of dopamine hydrochloride DA a kind of redox active catecholamine neurotransmitters coupled with catalytic ferric ions or copper could induce oxidatively DNA damage DNA damage involves oxidation of guanine to 8 oxoguanine 8 oxoG and DNA strand scission It proceeds through the reactive oxygen species ROS generation This paper proposed an electrochemical biosensor have investigated the role of Fe III and Cu II catalyzed oxidation of dopamine to induce oxidatively generated DNA damage and its prevention Incubation of DNA modified electrode with DA solution containing Cu II or Fe III has been shown change in the electrochemical properties and the oxidative damage to the DNA It was found that presence of Cu II and Fe III in solution caused damage to DNA The inhibitory effect of glutathione GSH and ascorbic acid AA on the DA mediated DNA damage has also been investigated using the biosensor In The present work a pretreated pencil graphite electrode PGE modified with multiwall carbon nanotubes MWCNTs and chitosan CHIT decorated with the ds DNA were prepared as a sensor DNA damage induced by radicals generated from the dopamine in the presence Of some transition metals DNA damage was detected by using differential pulse voltammetry and electrochemical impedance spectroscopy using K3 Fe CN 6 K4 Fe CN 6 The change in the charge transfer resistance Rp and decrease in the intensity of the guanine and adenine oxidation signals after incubation of the DNA biosensor in the damaging solution for a certain time was used as indicators of DNA damage Keywords DNA damage DNA based biosensor Dopamine hydrochloride Ascorbic acid Glutathione

علي اصغر انصافي

بهزاد رضايي

محمد سراجي، محمدتقي جعفري